Abstract: Effects of different furrow irrigation patterns, water and nitrogen supply levels on hydraulic conductivity and yield of maize were studied by orthogonal experiment design. Hydraulic conductivity of maize root and shoot was measured for stage of shooting, heading and late filling by transient method with High Pressure Flow Meter (HPFM) developed by Dynamax Corporation in USA in the maize field. The results showed that maximum root and shoot hydraulic conductivity was obtained in stage of shooting, its value was gradually smaller along with growing stage. The optimal combination was obtained by the yield and hydraulic conductivity of maize in this experiment, which the relative furrow irrigation patterns was alternate partial root-zone furrow irrigation pattern and the irrigation quota was 282mm, and the quantity of supply nitrogen was 240 kg?hm-2. It is obvious that alternate furrow irrigation repeating watering for growing root in drying rootzone, promoting root and shoot hydraulic conductivity increased, water fertilization use and transport efficiency improved, and higher nitrogen accumulation in plant and yield of maize was obtained.