Abstract: Reconstruction of three-dimensional plant morphological structure is the basis for digital plant research. In this paper, ball B-spline curve and interpolation of B-spline surface were combined to realize geometric modeling of tobacco leaves with venation skeleton structure. In modeling process, 3D scanner and other measurement tools were firstly used to get 3D coordinates and thickness of veins according to principal morphological structure of tobacco leaves. And then veins models were generated with ball B-spline curve to get the morphological skeleton, through synthesis with leaves surface which was described with interpolation of B-spline surface. The final models were completed to realize 3D reconstruction and reality exhibition of tobacco leaves. The results show that the leaves models generated by this method are more realistic, and the method can provide a new technique for explicit modeling of plant leaves.