Abstract: In order to investigate the function of the agents on the process of composting and provide a theoretical basis of speeding up the brewery sludge composting and reducing odor production, flora DF-1 was inoculated to the brewery sludge and chicken manure pile. The parameters of composting and the dominant microbial of flora DF-1 were measured. The non-inoculated pile was taken as control. The results showed that the concentration of NH3 in two piles increased at initial and then declined in the trend. The concentration of H2S declined continually. The concentration of NH3 was still 0.39 mmol/m3 in composting maturity stage of no-inoculated pile, while it was 1.25 mmol/m3 in the composting section 10 d of inoculation pile, and then it could not be measured. The concentration of H2S was still 0.7 mg/L in the composting section 22 d of No-inoculated pile; it was 0.48 mg/L in the composting section 10 d of inoculation pile, and then it could not be measured. Flora DF-1 could reduce the pile moisture content, and its pile composting of TN were higher than that of no-inoculated pile, which indicated that flora DF-1 could not only reduce odor, but also make for the late dry granulation and compost product quality and efficient production of organic fertilizer. Lactococcus Lactis, Candida tropicalis and Trichoderma viride were the dominant bacterial of flora DF-1. It showed that there was an alternating process of evolution in the composting process of the dominant bacterial of flora DF-1.