Abstract: In order to extend the shelf life of Trichiutus haumela under cold storage, the influences of compound biopreservative and biopreservative combined with modified atmosphere packaging on the shelf-life of Trichiutus haumela stored at (4±1)℃ were studied with air packaged product as controls. The sensory evaluation values, total viable count (TVC), total volatile base nitrogen (TVB-N), pH value, the 2-thiobarbituric acid (TBA ) and the drip loss of Trichiutus haumela were used to evaluate the fish quality during the cold storage. The results showed that the shelf-life of air packaged product was about 4-5 days; mixing biopreservative (with 1.0% Chitosan and 0.4% Tea Polyphenols) had a significant bacteriostatic activity; and the drip loss with high density CO2 treatment was higher than that with other treatments. The compound biopreservative combined with modified atmosphere packaging with 60% CO2+10%O2+30%N2 had the best fresh-keeping effect that after the Trichiutus haumela storaged at (4±1)℃ for 20 days, the values of TVC and TVB-N of Trichiutus haumela were 5.59 lg(cfu/g) and 19.39 mg/100g, respectively, which meant the sample was still at the secondary freshness and the shelf-life could be extended to 20days, which was 4 times longer than that of the blank. These conclusions can provide reference for preservation and extend the shelf life of Trichiutus haumela.