Abstract: In order to reveal the influence of water and fertilization on load intensity of agricultural non-point source pollution in field, based on the estimation model of agricultural non-point source pollution in the field and experimental data, the load intensity of agricultural non-point source pollution in different water and fertilization in paddy field was calculated. The results showed that there was a positive exponential correlation between the irrigation amount and load intensity, and there was a positive correlation between the fertilization and load intensity. Secondly, the load intensity had obvious difference in different fertilization modes even with same fertilization level. Compared with other related research results and the present irrigation and fertilization condition in China, the agricultural non-point source pollution effects of the different irrigation and fertilization process in field were discussed. It was concluded that the irrigation and fertilization process in field had obvious influence on agricultural non-point source pollution, so taking the control measures to reduce the pollution was important, such as saving irrigation, scientific fertilization and reasonable adjust process of irrigation and fertilization in field etc.