Abstract: A variety of the multi-stripe and equal-interval laser melting treatments on the surface of 45 Steel samples were obtained using HLD1001.5 solid-state laser for studying the influence of scanning interval on the microstructure and abrasive wear resistance of 45 Steel by laser melting. The microstructure and properties of the samples were studied by SEM, Rockwell hardness tester and wear tester. The results showed that the hardened layer by laser melting was composed of melted zone, phase transformation zone and heat affected zone. The hardened layer was a martensite microstructure. The Rockwell hardness distribution was composed of left high value zone, left transitional zone, low value zone, right transitional hardness zone and right high value zone. The hardness at high value zone and low value zone was 58.1–59.6 HRC and 16 HRC respectively. Under the conditions of the experiment, the samples with the treatment of 4.5 mm scanning interval featured the best abrasive wear resistance.