Abstract: In order to realize rapid detection of reducing power of the antioxidants in the honey, the new approach was proposed to predict reducing power of three kinds of different nectariferous honey by electrochemical sensor in this work. Twelve features were extracted from electrochemical signal using cyclic voltammetry and preprocessed by normalization. Partial least square (PLS) model was employed to discriminate reducing power of honey. Experimental results indicated that the optimal model was achieved with r = 0.9425 and RMSECV = 0.0433 in calibration set; and r = 0.9392 and RMSEP = 0.0417 in prediction set. This work shows that electrochemical sensor combined with PLS has a significant potential in detection of reducing power of honey.