Abstract: Land use change will impact land ecology. The eco-efficiency of land use can reflect the ecology effect of land use by input-output analysis, which can minimize cost of land use and maximize ecological value. Firstly, CCR-I model based on date envelopment analysis (DEA) was selected，and the input-output index of land use was ascertained, then the ecological efficiency value of land use was calculated. Secondly, spatial distribution pattern of eco-efficiency of land use was analyzed by using the ecological efficiency value of land use. Thirdly, in order to insure DEA-efficiency of eco-efficiency, the inputs and outputs optimization of land use in 31 provinces were designed based on optimization principles. And the alterative value of regulation factor under fixed land area and GDP were acquired. The results indicated that the assessment of eco-efficiency of land use was feasible. There is a relationship between the spatial distribution pattern of eco-efficiency of land use and regional land use characteristics. The land use can achieve the DEA-efficiency of eco-efficiency by input-output optimization.