Abstract: In order to determine optimal packing methods for Pleurotus nebrodensis, the effects of packages including different thickness polyethylene(PE) film, micro-perforated film, vacuum packaging with PE film, PE film flushed with nitrogen on the quality of Pleurotus nebrodensis stored at 0℃ were investigated. Compared with other packaging methods, 0.035 mm PE film flushed with nitrogen was better for the quality of Pleurotus nebrodensis during cold storage, which got a higher score of sensory evaluation, and obviously reduced the activities of PPO，POD and the browing degree, delayed the aging process and membrane lipid peroxidation during storage. Meanwhile, compared with conventional PE film packaging, 0.035 mm PE film flushed with nitrogen could extend storage time by 10-15 days. The results can provide a theoretical basis for cold storage of Pleurotus nebrodensis.