Abstract: The development of embedded technology and wireless communication technology provide the necessary technical means for dairy cattle breeding. Based on analysis of the current method for dairy information collection and transmission, a new method was proposed which integrating RFID technology with wireless sensor network. The network architecture and communication protocol conversion process were investigated and the method was put into practice to develop the traceability information acquisition and transmission system for dairy cattle. Test results show that based on point to point communication method, the wireless sensor network could work in a range of 35 m with barrier and 75 m with barrier-free to effectively collect dairy information and transmit to the data center. The traceability information system can work real-time, steadily and reliability with package loss rate less than 5%.