Abstract: Ozone is a kind of effective disinfectant. When ozone is used in aquaculture water treatment, it must be dissolved in water. It is important to research ozone mixture equipment and dissolution technology for application of ozone in aquaculture. The present research concerned with the design, size parameters optimum and function measurement of ozone mixture equipment, and in structure design, ejector was used as a mixture parts and gas dissolving cane was used as dissolution component. Design method of Computation Fluid Dynamics (CFD) was utilized in 10 groups of ejector sizes, and the optimized main parameters of ejector were injecting coefficient as 0.7, injecting nozzle diameter as 23 mm, mixing tube diameter as 42 mm and tube length as 336 mm. Meanwhile, the performance of ozone mixture equipment was experimented, and ozone dissolution curve was obtained. Ozone could be dissolved easily and the ozone concentration of water could reach up to 0.15 mg/L. According to the experiment function of mixing, the quantity and concentration of ozone could be chosen in disinfecting water, destroying structure of organic substance, oxidizing ammonia, and condensing suspend substance.