Abstract: Rape harvest logistics loss is an important factor in the cost of production, the impact of rapeseed moisture content on the three main losses in single-step harvest and in multi-step harvest was studied in Foulum Research Centre of Denmark, and functions between moisture content and three main losses as well as total loss were established, and the effect of straw moisture content on harvest logistics loss was also investigated. The results show that with the lower moisture content, threshing loss and cleaning loss reduce, but too lower moisture content will increase the cutting header loss. The relationship between the total losses and moisture contents is significantly CUBIC function; In different moisture contents, the average losses of combine harvesting and multi-steps harvesting were respectively 16.358% and 18.771 and the former is less 2.4% than the latter; the minimum value of header loss is 0.976% in the three main losses and the average header loss is lower than the threshing loss and cleaning loss; The effect of straw moisture content on straw harvest logistics loss is not significant.