Abstract: In order to find out a convenient way of acquiring chlorophyll spatial distribution, the canopy spectral reflectance of corn seedlings was measured. The spatial distribution of canopy spectral reflectance and the chlorophyll content were analyzed on different growth stages. The results indicated that the spatial variability of the canopy chlorophyll content of corn was unstable at different growth stages. The variance was kept in a higher level over the growth stages. The correlation coefficients between chlorophyll content of corn canopy leaf and each vegetation index, NDVI, RVI, and spectral reflectance of 550 nm, were calculated. The chlorophyll content showed better correlation with NDVI and reflectance of 550 nm, while there was no high correlation between chlorophyll content and RVI. Focus on the relationship between reflectance of 550 nm and chlorophyll content, it was found that the correlation coefficient varied among -0.88~-0.69, and the highest value was observed at the early period of the five-leaf stage. It showed that the reflectance at 550 nm might characterize the chlorophyll content of corn canopy leaves. The method can be used to support the precision fertilization during the growth stages of corn.