Abstract: In order to investigate the characteristics of anaerobic digestion for food waste at the different organic load rate(OLR), a pilot scale (40 L) experiment based on anaerobic digestions of food waste was carried out in a fed-batch single phase reactor with increasing organic loading rate at normal temperature(27℃). When the organic loading rate was controlled between 3.89 kg/(m3·d) and 6.49 kg/(m3·d), the gas production rate was between 2.5 L/(L·d) and 4.5 L/(L·d), methane yields ranged from 300.59 L/kgVS to 488.52 L/kgVS, where average methane contents were between 54.05% and 56.04%, the VS removal rate was 55.12%-89.58%. The experimental results showed that during the anaerobic digestion of food waste at normal temperature, high methane yields and stable methanogenic process with controlling OLR between 3.89 kg/(m3·d) and 6.49 kg/(m3·d) can be obtained.