Abstract: In order to produce fuel-ethanol from the turfgrass, the mixing fermentation of hydrolysis was carried out by both the Pachysolen tannophilus and Saccharomyces cerevisiae. After the pretreatments of the powdering and soaking of alkaline oxide, the solid residues were decomposed by the mixing of both the cellulase and pectase. The results showed that the percentage of lignin removal reached 70% under the condition of solid-liquid rate 1:40 (w/w) of alkaline oxide, soaking time 48 h. The ethanol concentration reached 9.6 g/L and 11.7 g/L, respectively, after 30 h of fermentation. The conversion rates of sugar-ethanol were 25.7% and 38.1% (w/w), which had 61.2% and 74.7% of the theoretical value, respectively.