Abstract: The orthogonal test was adopted to optimize the factors such as the ratio of liquid and solid (L/S), the ratio of pectinase, cellulase and hemicellulase, the pH and the temperature, which influenced the hydrolysis of sweet sorghum bagasse (SSB). The optimum condition obtained from orthogonal test was applied to hydrolyze SSB pretreated by laccase or autoclaving. The results showed that the best condition for hydrolyzing samples within 72h was L/S=11:1, pH3.6, 30℃, pectinase:cellulase:hemicellulase=1:1:2, from which the hydrolyzing efficiency obtained was higher than solo cellulase, and enzymatic hydrolyzing efficiency could be enhanced by pretreatment. After autoclaving, SSB soaked in different solutions led to different yield of glucose. When KMnO4 was added into solutions respectively, the yield of glucose could be improved higher.