Abstract: In order to obtain an electrochemically active bacterium and research its electricity-producing performance, strain LY-2 was isolated from microbial fuel cell. According to its phenotypic, physiological and biochemical characteristics as well as the phylogenetic analysis based on the sequence of 16S rDNA, it shared the highest similarity to Dysgonomonas mossii and was identified as Dysgonomonas mossii LY-2. Results of cyclic voltammetry experiment showed that strain LY-2 was electrochemically active bacterium. The preliminary current generation experiment showed that LY-2 could use beef extract for electricity production. When beef extract was used as a fuel, MFC had the highest voltage (0.61 V) and power density (104.3 mW/m2). Based on these results, strain LY-2 seems to promise on research and application in MFC.