Abstract: In order to study the homogenate extraction of theasaponin from Camellia oleifera Abel. meal, effects of three independent variables, namely ethanol concentration, extraction time and liquid/solid ratio, on the yield of theasaponin were investigated by means of response surface methodology (RSM) combined with Box-Behnken experimental design based on the single factor experiments. Results showed that the optimal extraction conditions were as follows: the ethanol concentration was 78%, the extraction time was 40 s, and the liquid/solid ratio was 20 mL/g. Under these conditions, the yield of theasaponin was 21.09% ± 0.26%, which agreed with the predicted value. Compared with the traditional heat refluxing extraction method, the yield of theasaponin obtained from the homogenate extraction method had a bit of increase, the extraction time was shorter (6 h and 40 s, respectively), and the extraction temperature was lower (70℃ and 20~25℃, respectively). The homogenate extraction is an ideal method to extract theasaponin from Camellia oleifera Abel. meal.