马兰黄酮类化合物的提取及其抗氧化活性
Extraction and antioxidant activities of flavonoids from Kalimeris indica
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摘要: 为了探索以亚临界水萃取马兰黄酮类化合物的效果，以得率为指标，通过单因素和正交试验，优选其萃取工艺参数，并对所得黄酮类化合物的抗氧化性能进行测定。结果表明，在压力为5.0 MPa，温度160℃、提取时间25 min、水料比为20∶1 mL/g的条件下马兰黄酮类化合物的得率为9.01%。与水和乙醇回流提取法（马兰黄酮类化合物得率分别为5.12%和7.13%）相比，亚临界水萃取在提取时间和得率方面均有明显的优势，所得马兰黄酮类化合物在一定浓度范围内具有较强的清除自由基和抗氧化能力。研究结果为马兰黄酮类化合物的开发提供参考。Abstract: The subcritical water extraction technique for flavonoids from Kalimeris indica was studied, and the optimization of technological parameters were obtained by orthogonal experiments with flavonoids yield as index, and the antioxidant activities of flavonoids in the extracts were determined. The results were as follows: the flavonoids yield was 9.01% under the conditions of pressure 5.0 MPa, temperature 200℃, extraction time 25 min and the liquid-solid ratio 20 mL/g, while it was 5.12% and 7.13%, respectively by reflux extraction with water and ethanol. Compared with traditional method, subcritical water extraction method has obvious advantage in saving time and extraction rate. In addition, the flavonoids extracted from Kalimeris indica was found to be possessed power scavenging free radicals and antioxidant activities in the experiment concentration range. These results can provide references for the development of flavonoids extraction from Kalimeris indica.
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Keywords:
- extraction /
- optimization /
- agricultural products /
- Kalimeris indica /
- flavonoids /
- antioxidant activity
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