黑莓籽油的超临界萃取及脂肪酸成分分析

Supercritical CO2 fluid extraction technology and fatty acid composition of blackberry seed oil

摘要: 为了获得高品质保健油脂，采用超临界CO2萃取黑莓籽油，以气相色谱-质谱（GC-MS）对黑莓籽油脂肪酸成分进行分析。样品最佳粉碎粒度60目，超临界CO2萃取适宜工艺条件为：萃取压力20 MPa，分离罐压力10 MPa，萃取罐温度45℃，萃取时间30 min，萃取得率为(17.73±0.19)%。GC-MS检测结果显示黑莓籽油中含有丰富的不饱和脂肪酸，尤其是亚油酸、油酸、亚麻酸，质量分数分别为58.04%、11.76%、8.38%，占总脂肪酸的78.18%。研究结果为黑莓籽的综合开发加工利用提供了参考。

Abstract: In order to obtain high-quality healthy oil, supercritical CO2 fluid extraction was used to extract oil from blackberry seeds, and GC-MS was used to analyze the fatty acid composition of the oil. The optimum extraction parameters were confirmed as follows: material grind degree 60 mesh, extraction pressure 20 MPa, separation pressure 10 MPa, temperature 45℃, and extraction time 30 min. Under the above conditions, the yield of oil was (17.73±0.19)%. GC-MS analysis revealed that blackberry seed oil is rich in unsaturated fatty acid, especially linoleic acid (58.04%), oleic acid (11.76%) and linolenic acid (8.38%), accounting for 78.18% of the total fatty acid. The results can provide a reference for the comprehensive usage of black berry seeds.