Abstract: Pretreatment may increase the effect of dairy manure utilization, In this paper, dairy manure with water was separated using screw solid-liquid separator, fiber content of solid matter and separated liquid, heat value of solid matter and viscosity coefficient and C/N ratio of separated liquid were investigated. Solid matters were pressed into block by press mounding machine, providing fuel for temperature raising and maintaining in biogas fermentation. Anaerobic fermentation tests were carried and contrasted by using separated liquid and integrated original dairy manure. Results showed that methane-producing rate could be improved and hydraulic retention time decreased for separated liquid fermentation. Gas-producing rate of separated liquid increased by 32.68% than that of original dairy manure when materials VS were 4.86% and 4.98% with 30% inoculums at (35±2)℃, respectively. The results can provide references for reclamation of dairy manure in high-cold areas.