Abstract: To realize sustainable utilization of cultivated land and ensure dynamic balance of the cultivated land resource, it is necessary to analyze cultivated land requisition-compensation balance in Karst mountainous county area. With objective properties analysis included general planning of land utilization, cultivated requisition-compensation balance, relationships between human and land, environment, cultivated utilization effect, this study put forward the early warning index system of cultivated land requisition-compensation balance in Karst Mountainous area. Based on the index and regression analysis, the early warning of cultivated land requisition- compensation balance in Qianxi county of Guizhou province was analyzed. The results showed that the fluctuation of integrated index of the early warning of cultivated land requisition- compensation balance in Qianxi county of Guizhou province was intensive during 1996 to 2008. The intensity of early warning could be divided into five levels: no warning, light warning, medium warning, serious warning and severe warning. From 2009 to 2020, the integrated index of the early warning showed continuous reduce, and the cultivated land requisition- compensation balance would be in no warning level from 2009 to 2015, light warning level in 2016 and medium warning level from 2017 to 2020. This research could provide scientific basis for analyzing cultivated land requisition- compensation balance and guaranteeing food security in the region.