Abstract: In order to carry out the study of biological effects on extremely low frequency high-voltage pulsed electric field, a pulsed electric field generator based on potential fluctuation in plant was designed. The system can produce high-voltage pulse with extremely low frequency, wide pulse width and steep front. The amplitude of voltage is adjustable from 6 to 20 kV and the pulse frequency is adjustable from 0.1 to 15 Hz. Using the pulsed electric field with 100 kV/m, 1 Hz and 80 ms pulse width to treat germinating mung beans and maize seeds, the results indicated that the shoot length, root length and root number of mung beans and maize were significantly promoted. It was illustrated that the extremely low frequency high-voltage pulsed electric field based on potential fluctuations in plant had a significant biological effects. This paper laid the foundation for further study of biological effect on extremely low-frequency high-voltage pulsed electric field.