Abstract: Wireless sensor network (WSN) has unparalleled advantages and broad prospects in development of intelligent agriculture. The Gateway plays a important role as the bridge for linking WSN to external application system. Most of the researches currently are specialized in specific data logging and transmission; few of them have the capacity to manage the information of monitoring area. Taking general agricultural environment information monitoring system unrelated to application fields as the background, the paper proposed a Re-configurable Gateway Architecture (ReGA) based on Wince OS, that not only can transmit monitoring data and controlling instructions, but also serves as an integrated management platform for the property of network and environment factors through a visual interface, and provides a SMS warning for exceptional data. The flexibility of ReGA is embodied in supporting of dynamic configuration of environmental monitoring type, monitoring nodes’ properties, monitoring areas’ attributes. By defining interactive protocols with nodes and server, the interactive instructions and package parameters can be configured. Experiments prove that ReGA is highly reliable, and can be applied to various fields like farm, fishery, forestry and greenhouse flexibly.