Abstract: In order to explore the preparation method of biological film and its activity of anti-Aspergillus ?avus, the chitosan-based blend film was prepared from chitosan, soybean trypsin inhibitor extract (TI) and glycerol solution, and the properties of which were also investigated, including thickness, mechanical property, water vapor transmission, optical transmittance, solubility apparent structure and anti-A. ?avus activity. The results showed that the chitosan, TI and glycerol concentration got 18 mg/mL, 2 mg/mL and 12 mg/mL respectively, the blend films exhibited good physical and chemical properties, and the germination and growth of A. ?avus were strongly inhibited by blend films on peanuts.