Abstract: Measurement and control system of fermentation process is the basis of parameters measurement and optimal regulation in fermentation process, directly influencing the optimal control performance of the fermentation system. Considering the intelligent measurement of L-lactic acid fermentation process, an integration method of measurement and control system based on intelligent measurement for fermentation process was proposed. The system architecture and implementing method were presented. The intelligent measurement and control system using PXI bus based on virtual instrument technology for fermentation process was integrated, and its hardware and software design were given. By constructing soft-sensor model in this system, the unmeasurable variables of fermentation process can be effectively estimated and controlled on-line. It provides a new measurement and control system for fermentation process. The experiment results show that the glucose liquid consumption can be estimated on-line by using the system in the L-lactic acid fermentation process, and the real-time closed loop optimal fed-control can be realized, furthermore, the production of L-lactic acid increases by 23%, as compared with the traditional manual control.