不同糖质原料和菌株固态发酵制取乙醇的特性比较

    Comparison of ethanol production from different sugar feedstocks by solid state fermentation with two yeast strains

    • 摘要: 利用固态发酵技术直接将糖质原料转化为乙醇,过程简单且糖利用率高,已经成为近年来的一个研发热点。为寻求最适原料及菌种,该文对不同原料和菌株进行固态发酵产乙醇特性进行了比较研究:2种菌株为实验室自行筛选酵母菌株TSH和市场上常见的安琪酿酒酵母菌株AGL;3种糖料作物为甘蔗、甜菜和甜高粱。结果表明,无论对于TSH或者AGL,投入相同质量的原料,由于可发酵总糖初始含量的不同,最终乙醇产量最高为甜菜,甜高粱次之,甘蔗最低。为消除原料初始糖含量不同造成的影响,该文比较了消耗单位质量糖分所生成的乙醇质量,发现对于2种菌株甜高粱都具有最高的乙醇/糖转化率,说明甜高粱茎秆中糖分用于生成乙醇的比率较多,而用于维持酵母菌自身生长繁殖及副产物生成的糖分较少。比较2菌株的发酵能力,对于甜高粱TSH表现出较为明显的发酵优势:发酵周期比AGL缩短了近6 h,且乙醇/糖转化率略高于AGL;而对于甘蔗和甜菜原料,TSH的乙醇产率与AGL相差不大;说明自行筛选的TSH菌种对多种糖料发酵底物具有广泛适用性,具有良好的工业应用前景。该文对利用糖质原料固态发酵生产燃料乙醇工艺的工业化实现及改进具有一定的借鉴意义。

       

      Abstract: In order to find out suitable raw materials and strains for ethanol production by solid state fermentation, three kinds of sugar crops and two yeast strains were tested to compare their characteristics of ethanol/sugar conversion. One of the two strains investigated is TSH-CGMCC1949 strain, screened from sweet sorghum plant, and the other one is Angel yeast, generally used in current alcohol industry. The three kinds of sugar crops investigated were sugar cane, sugar beet and sweet sorghum stalks. The results showed that, with the same feedstock input quantity, the ethanol production from sugar beet was the highest, following by sweet sorghum, because of different initial sugar content. In order to eliminate the effect caused by varied sugar content, ethanol yield (g ethanol/g sugar consuming) was employed to compare the fermentation efficiency. Sweet sorghum stalks had attained the highest ethanol yield of the three materials. TSH performed some advantages through the contrast to AGL. TSH had shorted the fermentation period by nearly 6 h, and the ethanol yield was higher than that with AGL. The results indicated that, TSH had a wide substrate rage, applicable to many sugar crops in addition to sweet sorghum.

       

    /

    返回文章
    返回