Abstract: It is of significance to study the effect of land use change on ecosystem service values. Base on five dynamic data of land use in Huairou reservoir watershed, this paper analyzed the land use change and its consequent changes in ecosystem services value in the watershed from 1990 to 2008. The results showed that the main land use in the watershed was forest, and the area of forest and water exhibited an increasing trend. The area of farmland almost remained unchanged, while the area of unused land showed itself as a downtrend. The total ecological services value exhibited an increasing trend, and in 1995, 2000, 2005 and 2008 which increased by 0.57%, 1.58%, 2.46% and 2.88%, respectively compared with that in 1990. The forest and water were the land use types that made the greatest contribution to the total ecosystem services value of the study region. The accumulative ecosystem services value of two land use types accounted for over 80% of the total values in the watershed, indicating that those land use categories played important and remarkable roles in ecosystem services. And single ecosystem service value was in order of biodiversity maintenance>hydrological adjustment>gas regulation>climate regulation>maintain soil>recreation and culture>raw materials production>waste treatment and food production.