Abstract: According to the problems of long fermentation period and low gas production in the alpine region, a two-phase anaerobic fermentation device was designed for rural household biogas production in the alpine region. The fermentation parameters such as acid production, temperature and pH value of feed and hydraulic retention time were controlled during two-phase anaerobic fermentation. The relationship between the quantity of produced biogas during fermentation and the quantity of consumed biogas to maintain the biogas slurry temperature in most cold season in Harbin was discussed. Results showed that the highest methan production was 1.35 m3/(m3·d) using the two-phase anaerobic fermentation process, which was four times higher than the traditional process of 0.35 m3/(m3·d). There was 4.08 m3 daily balance biogas to satisfy lighting and cooking for three people family in January of winter, which could operate in alpine region year-round. In addition, the equipment structure is reasonable and perfect, which might replace the traditional digester. The research can provide a technical support and reference for developing new pattern of domestic biogas production in alpine region.