Abstract: For optimizing the ultrasound-assisted extraction technology of fat-soluble components from Periploca sepium Bunge, on the basis of single-factor experiments, ultrasonic power, solid-solvent ratio and ultrasonic time were selected as main influencing factors to analysis the effects on extraction results of fat-soluble components from Periploca sepium Bung by response surface analysis methodology. The main fat-soluble components extracted from Periploca sepium Bunge were detected by using GC-MS. The results showed that the optimum conditions for ultrasound-assisted extraction of fat-soluble components from Periploca sepium Bunge were as follow: under the room temperature of 20℃ and ultrasonic frequency of 20 kHz with petroleum ether as chemical reagent, ultrasonic assisted extraction time 70 min, ultrasonic power 730 W, material-solvent ratio 1:10 g/mL. Under these conditions, the actual extraction yield of fat-soluble components from Periploca sepium Bunge reached to 6.14%.