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胺基化磁性壳聚糖微球对苹果渣多酚的吸附条件优化

袁亚宏, 蔡露阳, 岳田利, 高振鹏, 赵旭博

袁亚宏, 蔡露阳, 岳田利, 高振鹏, 赵旭博. 胺基化磁性壳聚糖微球对苹果渣多酚的吸附条件优化[J]. 农业工程学报, 2012, 28(11): 279-286.
引用本文: 袁亚宏, 蔡露阳, 岳田利, 高振鹏, 赵旭博. 胺基化磁性壳聚糖微球对苹果渣多酚的吸附条件优化[J]. 农业工程学报, 2012, 28(11): 279-286.
Yuan Yahong, Cai Luyang, Yue Tianli, Gao Zhenpeng, Zhao Xubo. Optimization of adsorption conditions for apple polyphenols by aminated magnetic chitosan microspheres[J]. Transactions of the Chinese Society of Agricultural Engineering (Transactions of the CSAE), 2012, 28(11): 279-286.
Citation: Yuan Yahong, Cai Luyang, Yue Tianli, Gao Zhenpeng, Zhao Xubo. Optimization of adsorption conditions for apple polyphenols by aminated magnetic chitosan microspheres[J]. Transactions of the Chinese Society of Agricultural Engineering (Transactions of the CSAE), 2012, 28(11): 279-286.

胺基化磁性壳聚糖微球对苹果渣多酚的吸附条件优化

基金项目: 国家自然科学基金项目(31071550,31171721);农业部“948”项目(2011-G8-3);陕西省科技攻关(2009K01-20);西北农林科技大学基本科研业务青年项目(QN2009073)

Optimization of adsorption conditions for apple polyphenols by aminated magnetic chitosan microspheres

  • 摘要: 为分离苹果渣中的多酚物质,研究壳聚糖磁性材料对多酚物质提取的影响。采用化学共沉淀法制得Fe3O4磁核,反相悬浮交联法制得磁性壳聚糖微球,并进行胺基化改性;利用扫描电镜、激光粒度仪对微球进行表征。采用单因素试验和响应曲面法对磁性微球吸附分离苹果渣多酚的吸附条件进行了工艺优化。结果表明:胺基化磁性壳聚糖微球吸附苹果渣多酚工艺参数对多酚吸附率有显著影响,因素影响主次顺序为微球用量>摇床转速>吸附温度;胺基化磁性壳聚糖微球吸附苹果渣多酚的工艺参数为:微球用量0.25 g/mL,吸附温度45℃,摇床转速127 r/min,吸附率可达81.58%。壳聚糖与Fe3O4磁性物质相结合,对多酚物质具有良好的吸附性能,可以对多酚物质进行快速分离,解析后多酚得率可达78.73 %。研究结果为磁性材料吸附分离多酚类物质提供了理论参考。
    Abstract: The aim of this study was to provide a new and feasible way for separation apple polyphenols(AP), and to study the influence of AP by chitosan magnetic materials. Fe3O4 particles were synthesized by coprecipitation. Magnetic chitosan microspheres were prepared by inverse suspension cross-linking, and analized through SEM and laser particle size analyzer after aminated. Then, apple polyphenols were adsorbed using the aminated magnetic chitosan microspheres. The single-factor experiment and response surface method were applied to optimize the adsorption factors of AP. Process parameters of AP separation by aminated magnetic chitosan microspheres had significant effect on adsorption rate of AP. The influence order of factors was: usage of microspheres>shaker speed>adsorption temperature. The optimum adsorption conditions of AP by aminated magnetic chitosan microspheres were as follows: usage of microspheres 0.25 g/mL, adsorption temperature 45℃, shaker speed 127 r/min, and under this condition, the adsorption rate could reached to 81.58%. The combination of chitosan and Fe3O4 magnetic material had a good adsorption performance and quick separation of AP. The results of the research can provide a theoretical reference for the adsorption of AP by magnetic materials.
  • [1] 赵锋,杨兴华,薛培生,等. 世界苹果产业概论[J]. 落叶果树,2005(3):16-17.
    [2] Yolanda D G, Beln S V, Anna P L. Phenolic and antioxidant composition of by-products from the cider industry:applepomace[J]. Food Chemistry, 2009, 117(4): 731-738.
    [3] 李建新,王育红,潘治利,等. 苹果多酚的提取技术和应用研究[J]. 农产品加工,2007(12):62-65.
    [4] Auqustin Scalbert, Gary Williamson. Dietary intake and bioavailability of polyphenols[J]. American Society for Nutritional Science, 2000, 130(8): 2073-2085.
    [5] 牛鹏飞,仇农学,杜寅. 苹果渣中不同极性多酚的分离及体外抗氧化活性研究[J]. 农业工程学报,2008,24(3):238-242.Niu Pengfei, Qiu Nongxue, Du Yin. Separation of polyphenols from apple pomace based on different polarities and their antioxidative activities in vitro[J]. Transactions of the Chinese Society of Agricultural Engineering (Transactions of the CSAE), 2008, 24(3): 238-242. (in Chinese with English abstract)
    [6] Shoji T, Akazome Y, Kanda T, et al. The toxicology and safety of apple polyphenol extract[J]. Food and Chemical Toxicology, 2004, 42(6): 959-967.
    [7] Tsao R, Yang R, Xie S, et al. Which polyphenolic compounds contribute to the total antioxidant activities of apple?[J]. J Agric Food Chem, 2005, 53(12): 4989-4995.
    [8] Graziani G, D'Argenio G, Tuccillo C, et al. Apple polyphenol extracts prevent damage to human gastric epithelial cells in vitro and to rat gastric mucosa in vivo[J]. Gut, 2005, 54(2): 193-200.
    [9] 徐亚民. 天然抗氧化剂:苹果多酚的研究与应用[C]//齐庆中. 第十届中国国际食品添加剂和配料展览会学术论文集. 上海:中国食品添加剂生产应用工业协会,2006:109-113.Xu Yamin. The natural antioxidant: the development and application of apple polyphenols[C]// Qi Qingzhong. The 10th China international food additives and ingredients exhibition research papers. Shanghai: Chinese food additive production and application industry association, 2006: 109-113. (in Chinese with English abstract)
    [10] 唐传核,彭志英. 苹果多酚的开发及应用[J]. 中国食品添加剂,2001(2):41-45.Tang Chuanhe, Peng Zhiying. The development and application of apple polyphenol[J]. China Food Additives, 2001, (2): 41-45. (in Chinese with English abstract)
    [11] 郝少莉,仇农学. 苹果渣中多酚物质的提取技术研究[J]. 西北农业学报,2006,15(2):152-155.Hao Shaoli, Qiu Nongxue. Study on the extraction process of polyphenols from apple pomace[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2006, 15(2): 152-155. (in Chinese with English abstract)
    [12] Palenzuela B, Arce L, Mach A, et al. Bioguided extraction of polyphenols from grape marc by using an alternative supercritical-fluid extraction method based on a liquid solvent trap[J]. Analytical and Bioanalytical Chemistry, 2004, 378(8): 2021-2027.
    [13] 魏福祥,曲恩超. 超临界CO2从苹果渣中萃取苹果多酚的工艺研究[J]. 食品研究与开发,2006,27(7):60-63.Wei Fuxiang, Qu Enchao. Research of supercritical CO2 extraction apple polyphenol from apple pomace[J]. Food Research and Development, 2006, 27(7): 60-63. (in Chinese with English abstract)
    [14] 李涛,岳田利,袁亚宏. 苹果多酚提取的响应曲面法优化研究[J]. 食品科技,2007(8):118-121.Li Tao, Yue Tianli, Yuan Yahong. Opitimization of extraction conditions of apple polyphenol using response surface methodology[J]. Food Science and Technology, 2007(8): 118-121. (in Chinese with English abstract)
    [15] 王丽,李化,张钟. 微波辅助提取苹果皮中的多酚类物质[J]. 试验报告与理论研究,2007,10(6):25-28.Wang Li, Li Hau, Zhang Zhong. Microwave radiation extraction of polyphenols from apple peel[J]. Experimental Reports and Theoretical Researches, 2007, 10(6): 25-28. (in Chinese with English abstrat)
    [16] Yingrong Lu, Yeap Foo L. Identification and quantification of major polyphenols in apple pomace[J]. Food Chemistry, 1997, 59(2): 187-194.
    [17] Orekhova N M, Akchurin R S, Belyaev A A, et al. Local prevention of thrombosis in animal arteries by means of magnetic targeting of aspirin-loaded red cells[J]. Thrombosis research, 1990, 57(4): 611-616.
    [18] Lei L, Bai Y X, Li Y F, et al. Study on immobilization of lipase onto magnetic microspheres with epoxy groups[J]. Journal of Magnetism and Magnetic Materials, 2009, 321(4): 252-258.
    [19] 徐翠香,仓理,高强. 复合磁性壳聚糖微球对BSA的吸附机理研究[J]. 云南化工,2008,35(1):12-16.Xue Cuixiang, Cang Li, Gao Qiang. Study on the adsorption mechanism of chitosan magnetic microspheres on BSA[J]. Yunnan Chemical Technology, 2008, 35(1): 12-16. (in Chinese with English abstrat)
    [20] Liu J W, Zhang Y, Chen D, et al. Facile synthesis of high-magnetization γ-Fe2O3/alginate/silica microspheres for isolation of plasma DNA[J]. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2009, 341(1/3): 33-39.
    [21] Liao M H, Chen D H. Immobilization of yeast alcohol dehydrogenase on magnetic nanoparticles for improving its stability[J]. Biotechnology Letters, 2001, 23(20): 1723-1727.
    [22] 洪爱真. 胺基化壳聚糖微球吸附阴离子偶氮染料的研究[D]. 福建:福建师范大学化学与材料学院,2003.Hong Aizhen. Research on Adsorption of Anion Azo Dyes by Aminated Chitosan Microspheres[D]. Fu Jian: Fujian Normal University, 2003. (in Chinese with English abstract)
    [23] 李春阳,许时婴,王璋. 从葡萄废弃物中提取分离多酚类生物活性物质[J]. 食品科技,2004(6):88-93.Li Chunyang, Xu Shiying, Wang Zhang. Extraction the bioactive-products from the wastes of vint grape[J]. Food Science and Technology, 2004(6): 88-93. (in Chinese with English abstract)
    [24] 张文博,陈盛,夏启华,等. 胺基化壳聚糖树脂吸附分离茶多酚的研究[J]. 广州化学,2010,35(1):22-27.Zhang Wenbo, Chen Sheng, Xia Qihua, et al. Study on adsorption of tea polyphenols with aminated chitosan resin[J]. Guangzhou Chemistry, 2010, 35(1): 22-27. (in Chinese with English abstract)
    [25] 杨晋青,叶盛权,郭祀远. 磁性壳聚糖微球的制备及其应用[J]. 现代食品科技,2008,10(24):1079-1082.Yang Jiqing, Ye Shengquan, Guo Siyuan. Review of Preparation and Application of Magnetic Chitosan Microspheres[J]. Modern Food Science and Technology, 2008, 10(24): 1079-1082. (in Chinese with English abstract)
    [26] Moo-Yeal Lee, Kyung-Jin Hong, Toshio Kajiuchi, et al. Synthesis of chitosan-based polymeric surfactants and their adsorption properties for heavy metals and fatty acids[J]. International Journal of Biological Macromolecules, 2005, 36(3): 152-158.
    [27] Li Liu, Yeping Li, Yu Li. Rapid N-phthaloylation of chitosan by microwave irradiation[J]. Carbohydrate Polymers, 2004, 57(1): 97-100.
    [28] 张海真. 磁性壳聚糖微球的改性表征及吸附行为研究[D]. 湖北:中南民族大学化学与材料科学学院,2008.Zhang Haizhen. Modification and Characterization of Magnetic Chitosan Microspheres and Adsorption Behavious[D]. Hubei: College of Chemistry and materials of South-Central University for Nationalities, 2008. (in Chinese with English abstract)
    [29] Huang J H, Huang K L, Liu S Q, et al. Adsorption properties of tea polyphenols onto three polymeric adsorbents with amide group[J]. Journal of Colloid and Interface Sciencem, 2007, 315(2): 407-414.
    [30] 罗志敏,薛丽群,张婷,等. 珠状壳聚糖树脂对绿茶中茶多酚的吸附研究[J]. 食品科学,2009,30(1):86-89.Luo Zhimin, Xue Liqun, Zang Ting, et al. Study on adsorption of tea polyphenols from green tea onto beaded chitosan resin[J]. Food science, 2009, 30(1): 86-89. (in Chinese with English abstract)
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出版历程
  • 收稿日期:  2011-09-26
  • 修回日期:  2012-04-08
  • 发布日期:  2012-05-31

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