Abstract: The aim of this study was to provide a new and feasible way for separation apple polyphenols(AP), and to study the influence of AP by chitosan magnetic materials. Fe3O4 particles were synthesized by coprecipitation. Magnetic chitosan microspheres were prepared by inverse suspension cross-linking, and analized through SEM and laser particle size analyzer after aminated. Then, apple polyphenols were adsorbed using the aminated magnetic chitosan microspheres. The single-factor experiment and response surface method were applied to optimize the adsorption factors of AP. Process parameters of AP separation by aminated magnetic chitosan microspheres had significant effect on adsorption rate of AP. The influence order of factors was: usage of microspheres>shaker speed>adsorption temperature. The optimum adsorption conditions of AP by aminated magnetic chitosan microspheres were as follows: usage of microspheres 0.25 g/mL, adsorption temperature 45℃, shaker speed 127 r/min, and under this condition, the adsorption rate could reached to 81.58%. The combination of chitosan and Fe3O4 magnetic material had a good adsorption performance and quick separation of AP. The results of the research can provide a theoretical reference for the adsorption of AP by magnetic materials.