Abstract: To remove furfural in bio-oil, bamboo charcoal obtained by pyrolysis was introduced as adsorbent. The structure property of bamboo charcoal was measured, and the adsorption property was investigated by Langmuir and Freundlich isothermal adsorption equation. The results showed that the bamboo charcoal dominant with micropore contained various alkali oxygen-functional groups (such as -OH, -COOH), aromatic compounds and aliphatic compounds. Bamboo charcoal exhibited high adsorption capacity and removing efficiency, and the removing rate was over 95% with 10 g/L furfural and 200 g/L adsorbent dose at normal temperature. The adsorption was mainly attributed to the dispersion force. The adsorption process could be described with Freundlich isothermal adsorption equation. This research provides a new economic way for the high quality utilization of bio-oil.