Abstract: To explore the applied effects of lignocelluloses organic compounds on controlled-release fertilizer, three kinds of controlled-release urea coated with natural wood flour (CRU20, CRU25, CRU30) was examined by water-extraction method. The effects of temperature, pH and water-fertilizer ratio on the nutrient release characteristics of controlled-release urea were studied. The results showed that: 1) Controlled-release urea exhibited slow releasing performance, and the theoretical slow-release period of CRU20, CRU25, and CRU30 was 36, 54, and 121 days respectively, and the nitrogen-release model changed with the amount of coated materials; 2) Temperature and pH affected the nitrogen release rate greatly, while the impact of water-fertilizer ratio on nitrogen release rate was not obvious; 3) The release pattern of controlled-released urea coated with natural wood flour was failure mechanism. Controlled-release urea coated with natural wood flour provides a reference for applications.