Abstract: In order to improve extracting yield of collagen from black fungus, the optimum conditions for collagen extracting process with enzyme methods were investigated. The method of single factor experiment and polybasic quadratic regression orthogonal design model were used to analysis the effects of these factors. The results showed that , within limits, the extracting yield positively related with pH value and the ratio of liquid and material, negatively related with additive amount and extracting time. The optimized processing parameters were as follow: trypsin, enzymolysis pH value 8.0, ratio of liquid and material 52.5:1 mL/g, enzyme additive amount 8%, enzymolysis time 2 h. With these conditions, extracting yield of collagen from black fungus could reach up to 1.091%. The results can provide a reference for extracting plant collagen.