Abstract: In order to explore major mechanical properties soybean seed under different moisture content and disclose the damage mechanism of soybean seed during threshing and transportting, Shennong-12 soybean was seleted as test materials, major mechanical properties such as different forms of damage, elastic modulus, rupture strength and compression work for test index, the static compression test were carried out by means of universal bio-material tester with five levels of moisture content ranging from 6.84% to 21.37% and three kinds of stress direction. The relationship between moisture content and elastic modulus, moisture content and rupture strength, moisture content and compression work were researched. The results have important significance to well improvement structure and technology parameters of thresher and transporter of soybean seeds.