Abstract: It is the basis to prevent and resolve the current increasingly serious land use conflict effectively that identifying potential land use conflict scientifically. Taking Shenbei New District of Shenyang City as an example, a cultivated land multi-objective suitability evaluation system for cultivation and construction was designed, an identification matrix was established to identify potential land use conflict areas, and its application in basic farmland designation was studied. The results showed that the total cultivated land was divided into nine zones, the 2 type zones were potential land use conflict zones, account for 71.10% of the total amount of cultivated land; 3 type zones were non-conflict zones with cultivation advantage, account for 16.33%; 3 type areas were non-conflict zones with construction advantage, account for 9.29%; The other type zones were non-conflict zones, where cultivation and construction suitability were both lower. Combining conflict identification results with the agricultural land classification, basic farmland in Shenbei New District was designated. The research can provide a technical support for prevention and solution of land use conflicts and policy decision for cultivated land protection.