Abstract: To resolve the high energy consumption present in ventilation system and vast weight loss in fruits and vegetable during fresh-keeping transportation, an experimental platform with frequency conversion ventilation system and controlled atmosphere system was established. The frequency of ventilator was altered to research the regulating characteristics of variable air volume on working performance of devices including liquid nitrogen injecting equipments, refrigerator, ultrasonic humidifier and air interchanger. To meet the fruit and vegetable fresh-keeping requirements and minimize energy consumption, the ventilator frequency was optimized for improving the work performance of the devices. Frequency conversion ventilation system was designed, and the energy consumption of fixed frequency ventilation system and measurement were compared. The results indicated that frequency conversion ventilation system could meet fruit and vegetable fresh-keeping requirements, and the energy consumption of which was less than fixed frequency ventilation system at 15.2%. The energy consumption of frequency conversion ventilator was less than fixed frequency ventilator at 81.8%. The design on frequency conversion ventilation system could provide a reference for the improvement of fresh-keeping transportation technology with controlled atmosphere.