Abstract: According to unreasonable parameters about structure and operation of soybean-lifter making the loss of header increased, based on measured the plant properties and the mechanical properties of soybean stalk, the soybean plant model and lifter were established by CAD-software Solidworks and the virtual dynamic prototype was established by using the dynamic simulation software-ADAMS. The motion of soybean stalk's lifting process was simulated in ADAMS/View. Virtual orthogonal test of the two factors with three levels was employed to study the optimization of structural and operation parameters which affected lifter properties. The travel speed and the lifter angle were selected as factors, and the impact-force of x(direction of operation), y(vertical upward), z(horizontal to left direction) axis between soybean plant and lifter was selected as response function to represent the performance of lifting and the loss of header by using software Design-Expert 6.0.10. And virtual optimization results were verified by field test. The results indicated that the impact-force of x, y, z axis was 52.31, 73.52 and 95.87 N respectively when the working velocity was 0.9m/s and the lifter angle was 22°in the virtual tests and the performance of lifting was the best and the loss rate of header was 1.19% .The paper provides theory references for designing the soybean lifter.