Abstract: Based on locust's phototactic mechanism and locust's shocking characteristic stimulated by vibratory stimulation, the comparative test of locust's phototactic response to light-vibration coupling and spectral illumination was carried out by utilizing LED light source, vibration stimulation device and locust's behavioral experiment equipment. The results showed that spectral illumination and vibration stimulation coupling effects on locusts had the push-pull stimulation effect, vibration stimulation enhanced the increasing phototactic effects of locusts, and increased locust's phototactic response, while spectral light determined locust's phototactic induction effect. Spectral light and vibration stimulating locust's different sensory receptors initiated the distinction of locust's neural processing and sensitive response, causing that the cycle vibration mode(vibration frequency was 50Hz, vibration time was 400ms and vibration intermittent stop time was 1s) and higher vibration intensity and the enhancing effects of locust's phototactic response intensity was better in the cycle vibration mode coupled with violet light. Light-vibration stimulating locust could lead to the physiological effect, and the initial stimulation effect generating the physiological effect of locust was the stronger with the stronger energy of light and vibration. The difference of locust's sensitive response point to light and vibration, the attenuation properties of light and vibration transmission, locust's sensitive selective tuning would influence the increasing degree of locust's phototactic effects stimulated by light-vibration coupling. So through combining the strength threshold of vibration scaring locust's response with the optimal regulative mode of vibration stimulating locusts can realize vibration stimulating with moving and approaching step by step in scope of locust's response to spectral illumination and locust's phototactic effect could be enhanced effectively. These results could provide the technical support for the implementation of photoelectric induction equipment of locust.