Abstract:
Abstract: The effect of electric field on crop seeds is one of the popular issues in electric field biological effects. Previous studies were focused in high voltage electrostatic field (HVEF), few researches on pulsed electric field (PEF). In this study the germinating mung beans were treated in extremely low frequency PEF with 1 Hz and HVEF, and their growth indicators such as fresh quality, shoot length and root length, and associated physiological variables such as spontaneous luminescence, soluble protein content, the activity of superoxide dismutase (SOD) and peroxidase (POD) were investigated. The results showed that fresh quality, shoot length and root length of germinating mung beans treated in PEF with 1 Hz were significantly promoted, while the effect of HVEF with the same strength was not obvious. The mechanism study indicated that the PEF and HVEF had some influences on oxidative and protein metabolism of germinating mung beans, and decomposition of storage protein in the initial stage of germination and synthesis of new protein in later stage of germination were promoted by the PEF, while HVEF promoted the synthesis of protein in later stage of germination. The study also found that the PEF and HVEF can activate SOD induced by superoxide anion free radical and promote the synthesis of POD. The cause of the difference about effect of extremely low frequency PEF and HVEF on germinating crop seeds was also discussed in this paper. It is concluded that same intensity extremely low frequency pulsed electric field (PEF) with 1 Hz and HVEF can promote crop seed germination and growth of the crop, however, the PEF with 1 Hz is more effective than HVEF.