Abstract: Slope farmland is an important part of cultivated land resources in hilly areas of China. Identifying the spatio-temporal distribution and evolution characteristics of slope farmland resources is of great significance for rational planning and utilization of slope farmland resources and water and soil ecological environment construction. In this paper, using the land use data for 7 time points from 1980 to 2015, based on GIS spatial analysis technology, dynamic degree model, kemel density estimation and landscape pattern index model, the spatio-temporal distribution pattern and evolution law of slope farmland in Yunnan for nearly 35 years were quantitatively analyzed. The results showed that: 1) the area of slope farmland in Yunnan was 472.55×104 hm2, accounting for 69.79% of the cultivated land area, and the average slope was 15.62°. The relationship between the area ratio of slope farmland in different agricultural divisions was: Southwestern Yunnan>Northeastern Yunnan>Northwestern Yunnan>Southern fringe>Western Yunnan> Southeastern Yunnan>Central Yunnan, and the average slope size relationship was: Northwestern Yunnan>Southwestern Yunnan>Northeastern Yunnan>Western Yunnan> Southern fringe >Southeastern Yunnan > Central Yunnan. 2) In the past 35 years, significant dynamic transfers had occurred in slope farmland, forest land, grassland and paddy fields, but the process of transfer in and out was generally balanced, and the area of slope farmland has a slight increase trend during the process of land use transfer. 3) The slope of most slope farmland was more than 8°, and the proportion of slope farmland with slope >15° was 78.54%. The area of slope farmland with different slopes in the past 35 years had a dynamic process. It showed an increasing trend for the slope farmland area with slope <15°, while decreasing trend for the slope farmland area with slope >15°. The area of slope farmland with different slopes has the dynamic process of "decreased→increased→decreased" or "decreased→increased→ decreased→increased", and the fluctuation degree of >25° slope farmland was the largest. 4) With the evolution of spatio-temporal distribution of slope farmland in the past 35 years, the distribution characteristics of kemel density value in slope farmland have changed, but the change range was small. The distribution of slope farmland in most areas was in low-density value, and the area of high-density value was the smallest. Slope farmland distribution clusters were represented by four significant distribution zones: the Lincang distribution zone, the Lincang-Xishuangbanna distribution zone, the Pu'er distribution zone and the Zhaotong distribution zone. 5) Among the eight types of land use, the landscape dominance of slope farmland was in the middle position, and the fragmentation characteristics of slope farmland were the most significant among all land use types. In the past 35 years, the degree of fragmentation of slope farmland landscape has decreased, and the degree of contiguous use of slope farmland has been strengthened. The research results can provide theoretical support for the rational planning and utilization of sloping farmland and soil and water ecological environment protection in Yunnan.