Abstract: In order to understand the relationship between greenhouse gas emission of layer-hen manure and its effects, four sorts of different disposal methods were implemented. Layer-hen manure was put into a closed bin composting system and the gas sample was obtained through a valve in the closed bin. This experiment took place between September 25, 2001 and October 24, 2001. Gas samples were analysed for concentration of carbon dioxide(CO₂), methane(CH₄), and nitrous oxide(N₂O). Combining the measured data of temperature, humidity, wind speed, and organic matter content, the results showed that carbon dioxide emission flux is very big under negative pressure ventilating, and has a correlation with temperature. Methane emission flux is lower because oxygen content is high in layer-hen manure. Under conditions of higher oxygen content the primary resource of nitrous oxide is nitration.