低温胁迫对圆叶决明的伤害及施用Ca肥防护效果的研究

翁伯琦; 江福英; 应朝阳; 方金梅; 李延

低温胁迫对圆叶决明的伤害及施用Ca肥防护效果的研究

Effect of chilling stress on the injury of Chamaecrista rotundifolia and protective function of applying calcium fertilizer

摘要

摘要: 圆叶决明在0℃低温下分别胁迫6、12、18、24 h后，叶片叶绿素总量较对照(25℃)下降21.89%、26.04%、28.16%、29.31%，可溶性蛋白质含量、H₂O₂含量和·O^-₂净产生速率随胁迫时间增加，活性氧清除酶SOD的活性随胁迫时间先上升后下降。施Ca²⁺处理可明显降低圆叶决明叶片·O^-₂净产生速率、H₂O₂含量和细胞质膜的电解质渗透率，同时提高了叶绿素含量和抑制活性氧的产生，其中以0.5 mmol/L浓度的CaCl₂处理效果为最佳，说明Ca²⁺对低温胁迫下圆叶决明的膜结构起有效的保护作用。

Abstract: After 6, 12, 18 and 24 hours' treatment with 0℃ chilling stress on Chamaecrista rotundifolia, the contents of chlorophyll (chl) decreased by 21.89%, 26.04%, 28.16% and 29.31% than CK(25℃), respectively, dissolubility protein content, H₂O₂ content and ·O^-₂ net generation rate increased with chilling stress time, the scavenging enzymes(SOD) activity increased at earlier stage and then dropped. The treatment of Ca²⁺ fertilizer under chilling stress could significantly decrease the net rate of ·O^-₂ generation, the content of H₂O₂ and the leakage rate of electrolyte, the contents of scavenging enzymes SOD, meanushile, the chlorophyll (chl) content increased. The effect of 0.5 mmol/L CaCl₂ treatment was the best. The results indicated that Ca²⁺ could protect the membrane structure of Chamaecrista rotundifolia under chilling stress.

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