Abstract: Molecular distillation can effectively remove the solvent residue of natural extracts and increase the safety of products. The refining of higher fatty alcohol extracts from rice bran by molecular distillation was studied. Results indicated that: under the pressure of 66.7 Pa, the contents of octacosanol and triacontanol in distillate increased as the temperature increased between 160℃ and 230℃, and were up to their peaks at 190℃, and then decreased; and under the temperature of 190℃, the contents of octacosanol and triacontanol in distillate increased as the pressure decreased between 26.7 Pa and 266.7 Pa, and reached the highest at 133.3 Pa. Temperature and pressure have significant effects on the refining of higher fatty alcohol extracts as key parameters during molecular distillation.