Abstract: The high intensity pulsed electric fields (HIPEF) method was assisted in extracting lycopene from tomato residual. The optimal processing conditions were acquired by the single-factor experiment and the orthogonal experiment, and then comparative extraction tests were performed among the HIPEF-assisted extraction method, the organic solvents extraction method, the ultrasonic-assisted extraction method, and the microwave-assisted extraction method. The results show that with the shortest time (16 μs), the highest extraction rate 96.7% by lycopene was achieved under the following conditions: extraction solvent being ethyl acetate, electric field intensity 30 kV/cm, electric pulse number 8, liquid-stuff ratio 9 mL/g, temperature 30℃ and single-stage extraction. Extraction rate of HIPEF-assisted extraction method is 2.4 times as much as that of the organic solvents extraction method, 1.04 times of the ultrasonic-assisted extraction method, and 1.23 times of the microwave-assisted extraction method. HIPEF-assisted extraction method is proved to be a new and fast way to extract lycopene from the tomato residual.