Abstract: Land-use regionalization is one of important contents of general land-use planning. Land-use functional regionalization was studied quantitatively based on niche theory in the paper. Firstly, the concept of land-use niche was proposed, and then land-use niche-fitness model was built based on the coupling relationship between requirement niche and realistic niche. Secondly, indices of land-use niche-fitness evaluation were constructed. Finally, taking the result of land-use niche-fitness evaluation as a criterion, land-use was regionalized based on GIS. Taking Jinan City as a study area, the results showed that the regionalization project was optimal and reasonable, andthe practicality of the model was verified. This work can provide a new technique for the similar research of land-use regionalization from a view of niche-fitness.