Abstract: In order to investigate the effect of TiO2 irradiated by 60Co-γ ray and loaded by activated carbon fibers on photocatalytic degradation of ethylene in the environment of cold storage, the TiO2/ACF photocatalytic materials deposited with nano-Ag (Ag-TiO2/ACF) irradiated by 60Co-γ ray were prepared, and effects of three different films on the photocatalytic degradation rate of ethylene were investigated in the simulated cold storage environment for horticultural products. Environmental Scanning Electron Microscope (ESEM)、Field Scanning Electron Microscope (FESEM) and X-ray diffractometer (XRD) were used to analyze the characterizations of the prepared Ag-TiO2/ACF. Results showed that: 60Co-γ ray could make Ag load on the surface of TiO2 well and TiO2 could be dispersed on the ACF uniformly after adding the dispersant of polyethylene pyrrolidone, which could effectively prevent the aggregation of the nano-particles. The average particle size of TiO2 became smaller, which could increase the efficiency of photocatalytic degradation of Ag-TiO2/ACF. The apparent rate constant of photocatalytic degradation reaction of Ag-TiO2/ACF film irradiated by 60Co-γ ray increased by 44% and 37% respectively compared to unirradiated TiO2/ACF film and irradiated TiO2/ACF film. The research can provide a basis for the application of TiO2 photocatalytic technology to degrade ethylene in fruit and vegetable cold storage.