Abstract: Germination treatment of brown rice can significantly increase the contents of nutrients and active substances such as γ-aminobutyric acid(GABA). To analyze the effect of germination conditions on the accumulation of GABA in indica black rice, the conditions such as immersion temperature, time, Ca²⁺ concentration in immersion solution, and germination temperature and time, etc. were optimized. Results show that the water regain and germination rate of indica black rice are affected by immersion temperature and time; the accumulation of GABA in the germination process is affected by Ca²⁺ concentration in immersion solution and germination temperature and time, etc.; the content of GABA under the conditions of immersion in 0.50 mmol/L CaCl₂ solution for 24 h at 28℃, and then germination for 30 h at 29℃ can reach 55.36 mg/(100 g), 3.9 times as that of before germination.