万楚筠, 胡双喜, 李文林, 邓乾春, 黄凤洪. 超临界CO2萃取脱皮菜籽饼粕油脂的可行性[J]. 农业工程学报, 2014, 30(9): 272-278. DOI: 10.3969/j.issn.1002-6819.2014.09.034
    引用本文: 万楚筠, 胡双喜, 李文林, 邓乾春, 黄凤洪. 超临界CO2萃取脱皮菜籽饼粕油脂的可行性[J]. 农业工程学报, 2014, 30(9): 272-278. DOI: 10.3969/j.issn.1002-6819.2014.09.034
    Wan Chuyun, Hu Shuangxi, Li Wenlin, Deng Qianchun, Huang Fenghong. Feasibility of extraction of oil from dehulled rapeseed cake using supercritical CO2[J]. Transactions of the Chinese Society of Agricultural Engineering (Transactions of the CSAE), 2014, 30(9): 272-278. DOI: 10.3969/j.issn.1002-6819.2014.09.034
    Citation: Wan Chuyun, Hu Shuangxi, Li Wenlin, Deng Qianchun, Huang Fenghong. Feasibility of extraction of oil from dehulled rapeseed cake using supercritical CO2[J]. Transactions of the Chinese Society of Agricultural Engineering (Transactions of the CSAE), 2014, 30(9): 272-278. DOI: 10.3969/j.issn.1002-6819.2014.09.034

    超临界CO2萃取脱皮菜籽饼粕油脂的可行性

    Feasibility of extraction of oil from dehulled rapeseed cake using supercritical CO2

    • 摘要: 为提高脱皮双低菜籽低温压榨饼的附加值,采用Box-Behnken响应面设计优化超临界CO2萃取脱皮双低菜籽低温压榨饼中油脂的工艺,研究萃取压力、温度和时间对油脂提取率的影响,并对萃取得到的油和粕的品质进行检测。结果表明:萃取温度对油脂提取率的影响显著(P<0.05);萃取压力和时间对油脂提取率的影响极显著(P<0.001),且交互作用非常显著(P<0.01);最佳工艺参数为萃取温度40℃、压力28 MPa和时间120 min,此条件下菜籽油的提取率为95.08%。超临界萃取的油色泽较浅,酸价与过氧化值都优于正已烷工艺,磷脂质量分数为0.051 mg/g约是正已烷工艺的1/32,维生素E和总酚含量较高;超临界萃取的脱脂粕蛋白溶解度高,颜色较浅,硫甙含量较少,品质明显优于正已烷脱脂粕。研究结果可为脱皮双低菜籽低温压榨饼的高值化利用提供数据参考。

       

      Abstract: Abstract: The double-low rapeseed contains ≤2% erucic acid in its oil and ≤35μmol/g of dry meal. The oil of double-low rapeseed contains very low levels of saturated fatty acids (6%), high levels of oleic acid (≥60%), and intermediate levels of linoleic and linolenic acids (20 %and 10 %, respectively). Because of its higher oil content and numerous agricultural advantages, double-low rapeseed is getting worldwide attention. The rapeseed oil, which was extracted by a traditional process with pre-press and solvent extraction, is of bad quality. Commercial hexane, which has been used as a solvent for crude edible oil extraction, has toxicological and environmental safety issues. Carbon dioxide in its supercritical state has properties and extraction capacities very similar to liquids. Supercritical carbon dioxide extraction is a new technique for oil extraction and is similar to conventional solvent extraction in that the material to be extracted is withdrawn from the substrate by using a solvent. In its supercritical state, carbon dioxide is the most commonly used supercritical solvent due to its nontoxicity, non-flammability, low cost, availability in bulk quantities, ease of removal from the extracted materials, and low critical temperature and pressure (31.06 ℃ and 7.38 MPa, respectively). To improve the additional value of oils and meals obtained from the cold press cake of double-low dehulled rapeseed, supercritical CO2 is used to extract double-low rapeseed oil and then the quality of the oil and meal obtained is studied. The affection of factors, which includes extraction pressure, temperature, and time effecting on oil yield, is investigated by Box-Behnken's response surface methodology. The model equation for predicting the optimum response values is established by Design Expert software. The adequacy of the model equation for predicting the optimum response values was effectively verified by the validation. The experiment result indicates that affection of extraction pressure and time on oil yield are more significant than extraction temperature. The interaction between extraction pressure and time has a very significant affection on oil yield. The optimum operation parameters of supercritical CO2 extraction are: extraction temperature 40℃, pressure 28MPa, and time 120min.In this optimal condition, the oil yield is 95.08% and is similar to the 95.30% predicted as maximum oil yield by the mathematical model under the condition of temperature 39.66℃ and pressure 27.50MPa with extraction time 120 min. The supercritical CO2 extraction of double-low rapeseed oil from dehulled cold press cake has a lighter color and better acid and peroxide value compared to n-hexane extraction. The phospholipid content of oil extracted with supercritical CO2 is 0.051mg/g, which is 1/32 of the n-hexane process, and the vitamin E and total phenolic content are 17.68ug/g and 5.17ug/mL respectively, which are both larger than the n-hexane extraction process. The meal of double-low rapeseed obtained from dehulled cold press cake with supercritical CO2 extraction, which has a superior quality compared to the meal obtained by n-hexane extraction, has lower glucosinolate content, high protein solubility, and better color. These study results can help utilize cold press cake of dehulled double-low rapeseed for high-value purposes.

       

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