复合菌剂提高果树枝条堆肥过程中酶活性

史龙翔; 谷洁; 潘洪加; 张凯煜; 殷亚楠; 赵听; 王小娟; 高华

doi:10.3969/j.issn.1002-6819.2015.05.034

复合菌剂提高果树枝条堆肥过程中酶活性

1.
西北农林科技大学资源环境学院，杨凌 712100
2.
西北农林科技大学理学院，杨凌 712100

基金项目: 国家自然科学基金（No.4087119，41171203）；公益性行业（农业）科研专项（201303094）；陕西省重大攻关项目（2011KTZB02-02）。

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- 收稿日期: 2014-11-18
- 修回日期: 2015-01-30
- 发布日期: 2015-02-28

Improving enzyme activity by compound microbial agents in compost with mixed fruit tree branches and pig manure during composting

1.
College of Resources and Environment, Northwest A&F University, Yangling 712100, China
2.
College of Sciences, Northwest A&F University, Yangling 712100, China

摘要

摘要: 为了探讨接种复合菌剂对果树枝条堆肥过程的影响，以猪粪和果树枝条为试验材料，研究了复合菌剂对高温好氧堆肥过程的温度、酶活性及微生物群落功能多样性的影响。结果表明，接种复合菌剂在堆肥前期提高堆肥温度，比对照处理高温期（高于55℃）持续时间多 3d，促进堆料的腐熟。接种菌剂还能有效地提高果树枝条堆肥过程中酶活性，纤维素酶、漆酶、锰过氧化物酶和木质素过氧化物酶活性在堆肥过程中分别比CK处理提高15.0%～19.8%、1.0%～11.0%、4.1%～26.8%、4.0%～22.2%。Biolog 微平板法测定结果表明，接种复合菌剂显著提高了堆肥中微生物的平均每孔颜色变化率（average well color development），并改变了高温期和降温期微生物对6大类碳源的利用；主成分分析表明，复合菌剂主要在高温期发挥作用，对堆料中微生物起分异作用的碳源主要为糖类和氨基酸类。
- 堆肥 /
- 酶 /
- 菌 /
- 果树枝条 /
- 酶活性 /
- 微生物功能多样性
Abstract: Abstract: The effects of compound microbial agents in compost with mixed fruit tree branches and pig manure during composting in a thermophilic aerobic state were examined .The research used the inoculants compound microbial agents and control compost. The changes of temperature, enzyme activity, and the microbial community functional diversity in compost materials during composting were determined. The results showed the temperature during the composting period was increased and the high temperature (above 55℃) maintaining period was three days longer. Besides, inoculation treatment and control treatment are all carried out to achieve compost maturity, what was more, when added to the compound microbial agents composting process, we can easily find that the number of the seed germination in inoculants treatment is obviously higher than it is in the control treatment (P<0.05), so we can find that this process caused the decrease of the pH value and the C/N ratio. Of course, they promoted the compost maturity. The activities of the enzymes were improved by adding compound microbial agents. The tested enzymes Cellulose, laccase (Lac), Manganese peroxidase (MnP), and lignin peroxidase (LiP) activities were higher in the treatment with microorganism agent than those in the control treatment by 15.0%-19.8%, 1.0%-11.0%, 4.1%-26.8%, and 4.0%-22.2%, respectively. The research showed that the activity of Cellulose, Laccase (Lac), and the lignin peroxidase (LiP) rose at the beginning of the experiment and then dropped gradually under the inoculants agent processing, and reached the peak value in the seventh day. Their numerical values were 0.96 mg/(g·d) and 72.6 U/g respectively. However, the Manganese peroxidase (MnP) and Lignin peroxidase (LiP) reached their peak in the second day. Their numerical peak values were 927.2 and 726.4 U/g respectively. In addition, the activity of Manganese peroxidase (MnP) which was dealt with the inoculation treatment and control treatment was rising a little at the last period, and it may be affected by the content of the nitrogen in the last period. The average well color development （AWCD） value of microbes in compost was improved significantly, and six kinds of carbon sources used by the microbes during the high temperature period and cooling period were also significantly changed by the inoculation compound microbial agents. The research showed that the use of the inoculation agent processing to sugar and polyamine is obviously higher than the control treatment in the high temperature period. They are improved by 50.0% and 77.0% respectively. However, the use of carboxylic acid, polymer, and the polyphenolic substance were obviously lower than the control treatment, and their utilization rates were reduced by 23.5%, 44.7%, and 39.3% respectively. In the cooling temperature period, the use of the inoculants agent processing to the other five carbon sources, except polyamine, were all higher than that in the control treatment (P<0.05). Principal component analysis clearly revealed that adding compound microbial agents plays a main role in the high temperature period during the composting, and the metabolic characteristics of micro flora during these two treatments were changing along with the composting process and the differentiation in the six utilizations of carbon sources were mainly sugars and amino acids.
- composting /
- enzymes /
- bacteria /
- fruit tree branches /
- microbial community functional diversity

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参考文献(38)

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复合菌剂提高果树枝条堆肥过程中酶活性

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出版历程

Improving enzyme activity by compound microbial agents in compost with mixed fruit tree branches and pig manure during composting

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出版历程

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